A DNA origami nanosensor based point of care device
In the context of the NCCR subproject “DNA Origami-based sensor for the multiplex detection of mRNA in cancer” building on collaborations that led to the development of optical DNA origami biosensors for microRNA detection and the detection of circulating tumor cells (CTCs) by SERS, the team is designing DNA origami-based sensors for the ultra-sensitive detection of mRNA in bulk solutions and in single cells including circulating immune cells. To this end, the team combines complementary expertise in oncology, biomarker discovery, DNA nanotechnology and nanophotonics.
We have thus developed a DNA origami nanosensor to detect specific miRNAs associated with breast cancer. Upon miRNA binding, the nanosensor produces fluorescence signals that are detected using an optical readout device. The nanosensor has been developed within the NCCR project and patented by UNIFR. Xemperia SA, a UNIFR spinoff, has exclusive commercial development rights.
An innovative portable fluorescence reader is thus needed, and will be developed for the detection of DNA origami for miRNAs linked to breast cancer. This will elevate the position of the test in the market beyond what was previously achievable. A collaboration with CSEM (who have a suitable fluorescent reader) will be established using funds from Innosuisse and CSEM.
A start-up that will develop a product has already been incorporated, Xemperia SA, a spinoff of UNIFR. If successful, this feasibility project, will set the stage for a Innossuisse project application of CSEM in collaboration with Xemperia.
The NCCR Bio-Inspired Materials has approved the Proof-of-Concept grant application (POC-07-2024), “A DNA origami nanosensor based point of care device”, for a total of 5’000CHF.
